The funds raised from the challenge will be split between the Rangers Charity Foundation and the Stoke City Community Trust.
Rangers Manager Ally McCoist commented: "On behalf of everyone at Rangers Football Club, I would like to wish Jim, Gordon and the team all the very best with their cycle.
"The Rangers Charity Foundation is very proud to support The Prince & Princess of Wales Hospice, ABF The Soldiers’ Charity and UNICEF and I am sure the 300 Mile Cycle Challenge will be a huge success.
"We look forward to welcoming Jim, Gordon and the rest of the team to Ibrox for our final home game of the season against Berwick Rangers at Ibrox."
Stoke City Manager Tony Pulis also offered words of encouragement to the team.
“It’s a fantastic effort, so they deserve all the support and encouragement they can get, both now in training for the event and throughout the ride itself,” added Pulis.
“I’m sure it will be an unforgettable experience. What will keep them going at times will be the knowledge they are raising money for a good cause.”
The team of cyclists will set off on May 1st and plan to arrive in Glasgow two days later, ahead of Rangers’ final home match of the season against Berwick Rangers.
Rangers fan Bertram explained: “Gordon and I came up with the idea of doing the cycle ride while on a trip to India last year. There is a long association between the two clubs which goes back to the time when the Loving Cup was presented to Rangers by Stoke.”
“We thought that raising money for the respective community schemes would be a great way for the two clubs to link up again and we hope to establish this as an annual event.
“The level of backing has been incredible from a whole host organisations and individuals, so much so that we have already raised our level of expectations,” he added.
“At the outset, we were hoping to raise £5,000 for each charity, but now we are aiming higher than that due to the support, something which will spur us on in the coming weeks.”
As well as arriving in Glasgow for Rangers’ final match of the season, the cyclists will also ride into City’s final home game of the season against Tottenham Hotspur eight days later.
To find out more about the challenge or sponsor the riders please click here.
The organisations who have already pledged their support for the bike ride are: Evans Halshaw, Citroen UK, The Co-operative Group, Menzies Hotels, Enterprise Rent-A-Car, Swinnerton Cycles, PREM Management and the Premier League Support.