The young Gers are without several of their regulars in this competition owing to the current set of international fixtures, but they will nonetheless be keen to get back to winning ways in their bid to make the knockout stages.
From the team which lost out narrowly to Stirling Albion a fortnight ago, in comes Sam Reid, Conor Owen, Ben Hutton, Ashton Patton and Zebedee Lawson.
It means Reid replaces Rydnn McGuire between the posts, behind a backline of Patton, Arran Kerr, Owen, Jack Wyllie and captain Zander Hutton.
In midfield, 16-year-old Luca Rankin retains his place alongside Ben Hutton.
Josh Gentles leads the line in attack, where he is supplemented by Kyle Glasgow and Lawson.
Among the substitutes in Hamilton is Greig Thackray, Jack Caldwell, Luke Ramage, Lyle Wark, Ethan Molloy, Cameron Fernie, Calum Young, Cormac Christie and Daniel Owoyomi.
Kick-off is 7:45pm and you can stay up to date with the action on X @RFC_Youth.
RANGERS: Reid, Patton, Kerr, Owen, Wyllie, Z. Hutton, Rankin, B. Hutton, Glasgow, Lawson, Gentles
SUBS: Thackray, Caldwell, Ramage, Wark, Molloy, Fernie, Young, Christie, Owoyomi
Rangers Academy is proudly sponsored by Carrick Packaging